- Title
- Pan-Cancer Analysis of Canonical and Modified miRNAs Enhances the Resolution of the Functional miRNAome in Cancer
- Creator
- Distefano, Rosario; Tomasello, Luisa; Ma, Qin; Nigita, Giovanni; Croce, Carlo M.; Rampioni Vinciguerra, Gian Luca; Gasparini, Pierluigi; Xiang, Yujia; Bagnoli, Marina; Marceca, Gioacchino P.; Fadda, Paolo; Laganà, Alessandro; Acunzo, Mario
- Relation
- Cancer Research Vol. 82, Issue 20, p. 3687-3700
- Publisher Link
- http://dx.doi.org/10.1158/0008-5472.CAN-22-0240
- Publisher
- American Association for Cancer Research
- Resource Type
- journal article
- Date
- 2022
- Description
- Epitranscriptomic studies of miRNAs have added a new layer of complexity to the cancer field. Although there is fast-growing interest in adenosine-to-inosine (A-to-I) miRNA editing and alternative cleavage that shifts miRNA isoforms, simultaneous evaluation of both modifications in cancer is still missing. Here, we concurrently profiled multiple miRNA modification types, including A-to-I miRNA editing and shifted miRNA isoforms, in >13,000 adult and pediatric tumor samples across 38 distinct cancer cohorts from The Cancer Genome Atlas and The Therapeutically Applicable Research to Generate Effective Treatments data sets. The differences between canonical miRNAs and the wider miRNAome in terms of expression, clustering, dysregulation, and prognostic standpoint were investigated. The combination of canonical miRNAs and modified miRNAs boosted the quality of clustering results, outlining unique clinicopathologic features among cohorts. Certain modified miRNAs showed opposite expression from their canonical counterparts in cancer, potentially impacting their targets and function. Finally, a shifted and edited miRNA isoform was experimentally validated to directly bind and suppress a unique target. These findings outline the importance of going beyond the well-established paradigm of one mature miRNA per miRNA arm to elucidate novel mechanisms related to cancer progression.
- Subject
- canonical miRNAs; cancer; patient; modified miRNAs; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1492433
- Identifier
- uon:53342
- Identifier
- ISSN:0008-5472
- Rights
- x
- Language
- eng
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